| Cat# | BDE-048 |
| Description | S-adenosylmethionine synthase (MAT) catalyzes the synthesis of S-adenosylmethionine (SAM) from ATP and methionine. SAM serves as a universal methyl donor in transmethylation reactions and is converted to S-adenosylhomocysteine upon methyl group transfer. Most SAM is produced in the liver. |
| Applications | Used in homocysteine assay kits to catalyze the conversion of methionine to SAH for downstream reactions. |
| Enzyme Commission Number | EC 2.5.1.6 |
| Form | Lyophilized powder |
| Source | Recombinant microbial proteins |
| Activity | 700 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to produce 1 μmol of S-adenosylmethionine per minute under specified assay conditions (37 °C, pH 8.0). |
| Molecular Weight | 46 kDa (SDS-PAGE) |
| Purity | ≥ 95% by SDS-PAGE |
| Optimum pH | 7.0-9.5 |
| Isoelectric Point | 4.7 |
| Storage | Store at -20°C for at least one year, or at -80°C for two years. |
| Notes | It can be resuspended in purified water containing 30% glycerol and stored at 4°C for approximately one year; After aliquoting according to usage, store at -20°C for up to two years; avoid repeated freeze-thaw cycles as much as possible. |
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