Enzymes for Producing Cholesterol Reagent Kit

Cholesterol is an organic molecule. It is a sterol, a type of lipid. Cholesterol is biosynthesized by all animal cells and is an essential structural component of animal cell membranes. Cholesterol is also implicated in cell signaling processes, assisting in the formation of lipid rafts in the plasma membrane, which brings receptor proteins in close proximity with high concentrations of second messenger molecules. According to the lipid hypothesis, elevated levels of cholesterol in the blood lead to atherosclerosis which may increase the risk of heart attack, stroke, and peripheral artery disease. Abnormally low levels of cholesterol are termed hypocholesterolemia. Research into the causes of this state is relatively limited, but some studies suggest a link with depression, cancer, and cerebral hemorrhage.

Cholesterol assay kit uses a simple method to quantify total cholesterol, free cholesterol, and cholesterol esters in mammalian samples. It also includes an easy method to separate HDL and LDL / VLDL cholesterol. In the cholesterol assay, cholesterol oxidase acts on free cholesterol to produce a chemical which reacts with a probe to generate color and fluorescence. Cholesterol esterase is used to hydrolyze cholesteryl ester into free cholesterol.

Creative Enzymes provides high-quality cholesterol esterase (EC 3.1.1.13), choline oxidase (EC 1.1.3.17), peroxidase (EC 1.11.1.7), and cholesterol oxidase (EC 1.1.3.6), which are raw materials used in the production of cholesterol reagent kits.

Cholesterol esterase (EC 3.1.1.13) is an important steroidal enzyme that catalyzes the hydrolysis of cholesterol esters into cholesterol and fatty acids. It is synthesized in the pancreas and is released in response to CCK in a fully active form. This enzyme has no effect on free cholesterol absorption but does dramatically lower cholesterol ester absorption, pointing to its key role in hydrolyzing cholesterol ester to free cholesterol and FA. Choline oxidase (EC 1.1.3.17) catalyzes the four-electron, two-step, flavin-mediated oxidation of choline to glycine betaine. This enzyme participates in glycine, serine, and threonine metabolism. Peroxidases (EC 1.11.1.7) are oxidoreductases that transform a variety of compounds via a free radical mechanism into oxidized or polymerized products. Specifically, peroxidase activity involves donating electrons to ferricyanides and ascorbic acid to break them into harmless components. Cholesterol oxidase (EC 1.1.3.6) is an enzyme that catalyzes the cholesterol and O2 into cholest-4-en-3-one and H2O2. This enzyme participates in bile acid biosynthesis.

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