| Cat# | DIA-138 |
| Source | Microorganism |
| Description | Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2. |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 1.1.3.6 |
| Activity | 12U/mg-solid or more |
| CAS No. | 9028-76-6 |
| Contaminants | Catalase < 1.0×10⁻¹% Cholesterol esterase < 1.0×10⁻²% |
| pH Stability | pH 5.0-10.0 (25°C, 20hr) |
| Michaelis Constant | 3.0×10⁻⁵M(Cholesterol) |
| Optimum pH | 7.0-8.0 |
| Optimum temperature | 60°C |
| Thermal stability | below 60°C (pH 7.0, 15min) |
| Stability | Stable at-20°C for at least 9 months |
| Stabilizers | Bovine serum albumin, amino acids |
| Inhibitors | Ionic detergents, Hg⁺⁺ |
| Synonyms | Cholesterol-O2 oxidoreductase; 3 beta-Hydroxy steroid oxidoreductase; 3β-hydroxysteroid: oxygen oxidoreductase; cholesterol: oxygen oxidoreductase; cholesterol oxidase; EC 1.1.3.6 |
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