| Cat# | DIA-548 |
| Source | Hexokinase: Recombinant from yeast G6P-DH: Recombinant from Leuconostoc mesenteroides |
| Description | High purity hexokinase (yeast) and glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) mixtrure for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| ECNumber | Hexokinase: 2.7.1.1 G6P-DH: 1.1.1.49 |
| CAS No. | Hexokinase: 9001-51-8 G6P-DH: 9001-40-5 |
| Optimum pH | 7.4 |
| Optimum temperature | 40 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | Hexokinase: One unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25 °C. G6P-DH: One unit of glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25 °C. |
| Storage | 2–8 °C |
| Synonyms | Hexokinase: hexokinase; ATP:D-hexose 6-phosphotransferase G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
| Concentration | Hexokinase: ~ 420 U/mL G6P-DH: ~ 210 U/mL |
| Specificity | Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine. Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+. |
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