| Cat# | DIA-175 |
| Source | Candida sp. |
| Description | The enzyme urate oxidase (UO), or uricase or factor-independent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5-hydroxyisourate: Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2 |
| Form | Freeze dried powder |
| Activity | GradeⅡ 4.0U/mg-solid or more (containing approx.20% of stabilizers) |
| CAS No. | 9002-12-4 |
| Isoelectric point | 5.4 |
| Synonyms | urate oxidase; uric acid oxidase; uricase; uricase; urate: oxygen oxidoreductase; EC 1.7.3.3; uricase II |
| Enzyme Commission Number | EC 1.7.3.3 |
| pH Stability | pH 7.0-11.0 (25°C, 20hr) |
| Michaelis Constant | 2.5×10??M (Uric acid) |
| Optimum pH | 8.5 |
| Optimum temperature | 40°C |
| Thermal stability | below 50°C (pH 8.5, 10min) |
| Stability | Stable at-20°C for at least one year |
| Stabilizers | Borate, EDTA, nonionic detergents |
| Inhibitors | Heavy metal ions, cyanide,various urate analogs |
| Contaminants | Catalase < 1.0% |
| Abbr | UO (Candida sp.) |
| Alias | UO; uricase |
| Applications | This enzyme is useful for enzymatic determination of uric acid in clinical analysis. |
| Appearance | White amorphous powder, lyophilized |
| Structure | 4 subunits per molecule (Reactive SH groups are present in the enzyme molecule) |
| Molecular Weight | approx. 120 kDa |
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