| Cat# | BDE-035 |
| Description | Diaphorase (DPH) mediates electron transfer using flavin as a cofactor and, when combined with tetrazolium salts, is widely used for signal amplification in NADH-based enzymatic assays. |
| Applications | Used for colorimetric signal amplification in NADH-related assay kits, widely applied in liver function, kidney function, and lipid analysis systems. |
| CAS No. | 9001-18-7 |
| Enzyme Commission Number | EC 1.6.5.2 |
| Form | Yellow amorphous lyophilized powder |
| Source | Microorganism |
| Activity | ≥ 600.0 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to consume 1 μmol of DCPIP per minute under specified assay conditions. |
| Molecular Weight | 28 kDa (SDS-PAGE) |
| Purity | ≥ 90% by SDS-PAGE |
| Optimum pH | 7.5 |
| Optimum temperature | 45°C |
| pH Stability | 4.5-9.0 (25°C, 16 h) |
| Thermal Stability | < 50°C (pH 7.5, 30 min) |
| Isoelectric Point | 5.8 |
| Km Value | 7.4×10^-4 M (NADH), 6.0×10^-2 M (NADPH) |
| Inhibitors | Fe3+, Mn2+, Cu2+ |
| Protectant | FMN, NAD(P)H |
| Storage | Store at -25°C to -15°C for 12 months to maintain at least 90% activity. |
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