| Cat# | DIA-834 |
| Descriptions | Recombinant Kex2 Protease is a calcium-dependent serine endoprotease that specifically cleaves peptide bonds at the carboxyl side of paired basic residues such as Lys-Arg and Arg-Arg, is widely used for processing fusion proteins and propeptide activation, and enables efficient removal of leader sequences from recombinant protein products. |
| Applications | Recombinant Kex2 Protease is applied in proprotein processing detection kits and fusion leader sequence cleavage assay reagent development. |
| Features | 1. Animal origin free: Recombinant Kex2 protease is no exogenous virus contamination, and any animal origin material is not used in the production process. 2. Stable quality: Mass production can ensure stable and continuous batch production. It is no difference between the batch and the product quality is stable. 3. High purity: Higher specific activity. Host protein residues is less than the limits of biological products. 4. Lyophilized powder: The product is lyophilized powder and is easy to store and transport. |
| CAS No. | 99676-46-7 |
| Enzyme Commission Number | EC 3.4.21.61 |
| Molecular Weight | Approximately 67.0±6.7 kDa |
| Enzyme Source | Pichia pastoris |
| Activity | ≥ 5.0 U/mg |
| Activity Definition | One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25°C with Boc-QRR-pNA as the substrate. |
| Storage | Recombinant kex2 protease should be stored under 2-8°C in sealed container. It is stable within 6 months. The product is stable by blue ice insulation transport. |
| Usage & Dosage | Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2+ or HEPES, 5 mM Ca2+. If it is not used immediately after dissolving, it is recommended to dissolve the powder with 20 mM (pH 5.2) NaAc-HAc and 2 mM Ca2+ buffer to make the final concentration is about 1-10 mg/ml, then make suitable packing as your requirement and store at -20°C. Reaction was carried out, using pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2+ or HEPES, 5 mM Ca2+ buffer. Note: The optimum reaction pH of the enzyme is pH 9.0 and the optimum stable pH is 5.0-6.0. |
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