| Cat# | BDE-025 |
| Specification | 3 KU, 100 KU, 500 KU, 1000 KU |
| Description | 3-α-Hydroxysteroid Dehydrogenase (3α-HSD) catalyzes the redox reactions of bile acids and androgens, playing a key role in liver function testing. |
| Applications | Used in total bile acid assay kits to enable enzymatic cyclic amplification detection of bile acids. |
| Enzyme Commission Number | EC 1.1.1.50 |
| Form | White amorphous lyophilized powder |
| Source | Microorganism |
| Activity | ≥ 55 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the production of 1 μmol of NADH per minute under specified assay conditions. |
| Molecular Weight | 30 kDa (SDS-PAGE) |
| Purity | ≥ 90% by SDS-PAGE |
| Optimum pH | 8.5-9.5 |
| Optimum temperature | 55°C |
| pH Stability | 6.0-9.0 (25°C, 16 h) |
| Isoelectric Point | 5.2 |
| Km Value | 3.6×10^-5 M (androstene), 4.7×10^-5 M (NAD+) |
| Inhibitors | Cu2+, Ag+, Hg2+, Zn2+, Fe3+ |
| Protectant | BSA |
| Storage | Store at -25°C to -15°C for 12 months to maintain at least 90% activity. |
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