| Cat# | BDE-002 |
| Description | Hexokinase (HK) is ubiquitous in animals, plants, microorganisms, and cultured cells. As the first key enzyme in glycolysis, it converts glucose to glucose-6-phosphate, a branch point between glycolysis and the pentose phosphate pathway. Clinical laboratories commonly use the glucose oxidase (GOD) and hexokinase (HK) methods; the HK method is the internationally accepted reference method due to its high specificity, precision, and accuracy, with minimal interference from common substances. |
| Applications | Serves as the core enzyme in glucose and ATP assays, generating quantitative signals through coupling reactions. |
| CAS No. | 9001-51-8 |
| Enzyme Commission Number | EC 2.7.1.1 |
| Form | White amorphous powder, lyophilized |
| Source | Microorganism |
| Activity | ≥ 100 U/mg-solid; ≥ 200 U/mg-protein |
| Unit Definition | One unit is defined as the amount of enzyme that catalyzes the formation of one micromole of NADH per minute at pH 8.0 at 37°C. |
| Molecular Weight | 54 kDs (SDS-PAGE) |
| Optimum pH | 8.0-8.5 |
| Optimum temperature | 45°C |
| Storage | Aliquot and store at ≤ -20 °C. |
| Download Datasheet: |  |
