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Recombinant Creatine Amidinohydrolase (CRH)

Cat# BDE-008
Description Creatinase (CRH) catalyzes the second step of the enzymatic creatinine assay by hydrolyzing creatine to sarcosine and urea.
Applications Used in combined enzymatic assays for creatinine and creatine, suitable for renal function biochemical test kits.
CAS No. 37340-58-2
Enzyme Commission Number EC 3.5.3.3
Form White powder, lyophilized
Source Recombinant
Activity ≥ 20 U/mg-protein
Unit Definition One unit causes the formation of one micromole of sarcosine per min at pH 7.5 at 37°C.
Molecular Weight 30 kDa (SDS-PAGE)
Optimum pH 7-9
Optimum temperature 50°C
pH Stability 5.0-10.5 (25°C, 16 h)
Thermal Stability < 50°C (pH 7.5, 30 min)
Inhibitors Ag+, Hg2+, Cu2+
Storage Store at ≤ -20 °C.
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