| Cat# | ENZD-RT02 |
| Specification | 10000 U; 40000 U |
| Unit Concentration | 200 U/μl |
| Description | AdvSTART Reverse Transcriptase is a reverse transcriptase that has been directionally modified from Reverse Transcriptase. It exhibits enhanced temperature tolerance, stability, and reverse transcription synthesis capabilities, making it suitable for high-temperature reverse transcription. AdvSTART Reverse Transcriptase is also particularly well-suited for one-step RT-qPCR reactions. |
| Notes | Warm-start RT enzyme. |
| Features | Excellent high and low template balance More suitable for self-built rapid one-step method system Higher stability |
| Applications | RT-qPCR self-built one-step method |
| Components | 5 × AccurSTART Buffer; AccurSTART Reverse Transcriptase (200 U/μl) |
| Quality Control | Excellent high and low template balance; Adaptation for quick programs; Higher stability |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
ENZD-RT02, a high-performance reverse transcriptase from Creative Enzymes, was evaluated for its ability to maintain consistent performance across varying template input levels. Benefiting from its hot-start characteristics, the enzyme minimizes non-specific reverse transcription and reagent consumption during low-temperature steps, such as reaction setup and thermal ramping.
As a result, ENZD-RT02 enables efficient cDNA synthesis even at low copy numbers, while maintaining comparable amplification plateau profiles to high-copy templates, demonstrating strong tolerance and balanced performance across a wide dynamic range. Data generated from internal reverse transcription performance studies.
IENZD-RT02 was engineered to support fast cDNA synthesis without compromising performance. Under identical amplification conditions, no significant Ct variation was observed between rapid reverse transcription (55°C for 2 min) and conventional protocols (55°C for 15 min).
This result highlights its efficiency as a robust reverse transcriptase, enabling flexible workflow design with both rapid and standard protocols. Data generated from internal RT efficiency evaluation studies.
ENZD-RT02 demonstrated excellent stability under various storage and handling conditions, including 50 freeze–thaw cycles in liquid nitrogen, storage at 4°C for 4 weeks, and storage at 37°C for 30 days. No significant performance decline was observed compared to the −20°C control, indicating consistent activity and reliable enzyme stability over time. Data generated from internal stability evaluation studies.
