| Cat# | DIA-426 |
| Description | β-Galactosidase is a tetrameric enzyme (135 kDa per subunit) containing sulfhydryl groups and multiple cysteine residues. It is widely used as a reporter enzyme for monitoring gene expression and in enzymatic assays such as ELISA. |
| Form | Powder |
| Activity | ≥ 400 U/mg protein |
| Enzyme Commission Number | EC 3.2.1.23 |
| Purity | ≥ 80.0% (SDS-PAGE) |
| Applications | Immunoassays (ELISA): β-Galactosidase can be conjugated to antibodies that specifically recognize target molecules for use in enzyme-linked immunosorbent assays. Reporter Enzyme: It serves as a reporter enzyme to monitor gene expression levels driven by specific promoters. Positive Control: It can be used as a positive control protein in assays involving anti-β-galactosidase antibodies. |
| Appearance | White amorphous powder |
| Function | β-Galactosidase hydrolyzes lactose into its monosaccharide components, glucose and galactose. Additionally, it catalyzes the transglycosylation of glucose to produce allolactose, which functions as an inducer of β-galactosidase in feedback regulation. |
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