| Cat# | DIA-823 |
| Descriptions | Recombinant Bovine Enterokinase Light Chain, His (rBoEK, His) is fused with a His-tag for simple purification, maintains strict substrate specificity for the DDDDK cleavage site, and enables efficient tag removal from various fusion protein substrates. |
| Applications | Recombinant Bovine Enterokinase Light Chain, His (rBoEK, His) is applied in affinity purifiable enterokinase detection kits and fusion protein cleavage assay reagent development. |
| CAS No. | 461-0158-1816 |
| Molecular Weight | Approximately 28 kDa, a single non-glycosylated polypeptide chain containing 241 amino acids, with 6 × His at C-terminus |
| Enzyme Source | Escherichia coli |
| Synonyms | Enterokinase, Serine Protease 7, Transmembrane Protease Serine 15 |
| Form | Sterile liquid | 50 mM Tris-HCl, pH 8.0, 0.5 M NaCl and 50 % glycerol. |
| Activity Definition | One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95 % completion at 25 °C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2. |
| Storage | Avoid repeated freeze-thaw cycles. Store at -20 to -70°C in the original packaging for up to 6 months from the date of receipt. After opening, store at -20 to -70°C under sterile conditions for up to 3 months. |
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