| Cat# | TRA-041 |
| Specification | 2KU,100KU,1000KU |
| Description | Malate dehydrogenase (MDH) acts as a coenzyme in the core reaction of transaminase (ALT/AST) diagnostic kits, enabling quantitative detection by catalyzing the interconversion of malate and oxaloacetate. |
| Applications | Used for the research, development, and large-scale preparation of aspartate aminotransferase reagents. |
| EC | EC 1.1.1.37 |
| Form | White, amorphous freeze-dried powder |
| Activity | ≥ 1300.0 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to consume 1 μmol of NADH per minute under the following reaction conditions. |
| Molecular Weight | 35 kDa |
| Optimum pH | 8.0-9.0 |
| Optimum temperature | 60°C |
| pH Stability | 4.0-9.0 (25°C, 16 h) |
| Thermal Stability | < 50°C (pH 7.5, 30 min) |
| Isoelectric Point | 5.61 |
| Km Value | 7.8×10^-5 M (Oxaloacetate), 1.2×10^-4 M (NADH) |
| Inhibitors | Hg2+ |
| Storage | Store at -25°C to -15°C for 12 months (retaining at least 90% activity) |
| Download Datasheet: |  |
