Bsu DNA Polymerase, large fragment, has 5′→3′ DNA polymerase activity but lacks 3′→5′ and 5′→3′ exonuclease activities. It possesses strand displacement activity and is commonly used in Recombinase Polymerase Amplification (RPA).
Applications
RPA-based isothermal amplification; RPA-mediated strand displacement DNA synthesis; random-primed labeling; second-strand cDNA synthesis; single dA tailing.
Product Overview
Product Components: 200 U: Bsu DNA Polymerase (10000U/mL): 20 μL 10x Bsu Reaction Buffer (Mg2+ plus): 1 mL 1000 U: Bsu DNA Polymerase (10000U/mL): 100 μL 10× Bsu Reaction Buffer (Mg2+ plus): 1 mL