| Cat# | DIA-824 |
| Descriptions | Recombinant Bovine Enterokinase Light Chain, Yeast (rBoEK, Yeast) is produced via yeast expression system with correct protein folding and high specific activity, exhibits excellent cleavage specificity and low non-specific degradation, and is suitable for processing pharmaceutical-grade recombinant proteins. |
| Applications | Recombinant Bovine Enterokinase Light Chain, Yeast (rBoEK, Yeast) is suitable for high-purity fusion protein processing detection kits and pharmaceutical grade protein assay reagent development. |
| CAS No. | 461-01-1816 |
| Molecular Weight | Approximately 43.0 kDa, a single glycosylated polypeptide chain containing 235 amino acids |
| Enzyme Source | Pichia Pastoris |
| Synonyms | Enterokinase, Serine Protease 7, Transmembrane Protease Serine 15 |
| Form | Sterile liquid | 50 mM Tris-HCl, pH 8.0, 0.5 M NaCl and 50 % glycerol. |
| Activity Definition | One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95 % completion at 25 °C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2. |
| Storage | Avoid repeated freeze-thaw cycles. Store at -20 to -70°C in the original packaging for up to 6 months from the date of receipt. After opening, store at -20 to -70°C under sterile conditions for up to 3 months. |
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