| Cat# | TRA-590 |
| Specification | 5μg(min) |
| Description | The catalytic domain of human JNK1 was expressed as an N-terminal GST fusion protein 69 kDa using an E. coli expression system. GST-JNK1 was purified by glutathione sepharose chromatography and then activated with His-tagged MAP2K4 and MAP2K7. The activated GST-JNK1 was re-purified using glutathione sepharose chromatography. |
| Applications | For use as a positive control in JNK1 detection assays for stress signaling research. |
| Synonyms | MAPK8, JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c |
| Source | E. coli |
| Molecular Weight | 69 kDa |
| Class | Serine/Threonine Kinase |
| Group | CMGC |
| Family | MAPK |
| Gene Name | MAPK8 | mitogen-activated protein kinase 8 |
| Sequence | 2-364 |
| Accession Number | NP_620634.1 |
| Tag | GST-Tag |
| Tag Location | N-terminal GST |
| Storage Buffer | 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35,1 mM DTT, 10% glycerol, pH 7.5 |
| Storage | Store at -80°C. Avoid repeating freeze-thaws. |
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