AdvSTART Reverse Transcriptase(Glycerol-free)

Cat#	ENZD-MDLyo01
Specification	10000 U; 40000 U
Unit Concentration	200 U/μl
Description	AdvSTART Reverse Transcriptase (Glycerol-free) is a formulation of AccurSTART Reverse Transcriptase developed without glycerol. This modification preserves the exceptional performance and stability characteristics of the original enzyme while enhancing its suitability for lyophilization through the complete removal of glycerol. This product is provided without excipients, allowing for their addition based on specific application requirements.
Notes	Glyceryl-free warm-start RT enzyme.
Features	Freeze-drying; Excellent stability
Applications	RT-qPCR is amenable to lyophilization, enabling the development of a simplified one-step assay.
Components	5 × AdvSTART Buffer; AdvSTART Reverse Transcriptase (Glycerol-free) (200 U/μl)
Shipping and Storage Conditions	Store at -30 ~ -15℃, transport at ≤0℃.
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Case Study

Case Study 1: Balanced Amplification Across High and Low Template Inputs

The performance of ENZD-MDLyo01 was evaluated across a range of template concentrations to assess amplification consistency under varying input conditions. Data generated from internal validation studies, demonstrating consistent amplification performance across varying template concentrations.

Fig. 1 Amplification performance of ENZD-MDLyo01 across different template input levels.

Case Study 2: Performance Evaluation under Rapid Amplification Protocol

The performance of ENZD-MDLyo01 was evaluated under a rapid amplification protocol and compared with a reference reverse transcriptase (ENZD-RT02) across high-, medium-, and low-template input levels. Data generated from internal validation studies, demonstrating comparable performance between ENZD-MDLyo01 and reference enzyme under rapid amplification conditions.

Table 2. Data results of the rapid protocol.

Case Study 3: Robust Stability Under Multiple Stress Conditions

The stability of ENZD-MDLyo01 was evaluated under multiple stress conditions, including repeated freeze–thaw cycles, long-term refrigerated storage, and elevated temperature exposure. Performance was assessed by comparing Ct values across two reaction systems, using a −20°C stored sample as the reference.
As a glycerol-free, lyophilization-compatible enzyme, ENZD-MDLyo01 exhibits excellent stability and reproducibility, supporting reliable performance during storage, handling, and transportation in diagnostic workflows. Data generated from internal validation studies, demonstrating consistent amplification performance under multiple stress conditions.