One unit (U) is defined as the amount of enzyme which produces 1 μmol of β-D-glucose-1-phosphate to β-D-glucose-6-phosphate per min at 37 °C and pH 7.0 under the conditions described below.
Storage
Below –20 °C
Enzyme Commission Number
EC 5.4.2.6
pH Stability
5.0–9.5
Michaelis Constant
2.3 × 10-4 M (β-D-glucose-1-phosphate)
Optimum pH
ca. 7.0
Optimum temperature
40 °C
Thermal stability
Below 45 °C
Inhibitors
Hg2+, Zn2+, Cu2+, Cd2+
Applications
For the determination of α-amylase and inorganic phosphate in clinical analysis.
Appearance
White lyophilizate
Molecular Weight
ca. 34 kDa (Gel filtration)
Notes
Expiration date: Liquid: stable at 37 °C for at least one week Powder: stable at 30 °C for at least four weeks