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Beta-Phosphoglucomutase (β-PGM) from E. coli

Cat# DIA-432
Source E. coli
Form Freeze dried powder
Activity ≥ 30 U/mg
Unit Definition One unit (U) is defined as the amount of enzyme which produces 1 μmol of β-D-glucose-1-phosphate to β-D-glucose-6-phosphate per min at 37 °C and pH 7.0 under the conditions described below.
Storage Below –20 °C
Enzyme Commission Number EC 5.4.2.6
pH Stability 5.0–9.5
Michaelis Constant 2.3 × 10-4 M (β-D-glucose-1-phosphate)
Optimum pH ca. 7.0
Optimum temperature 40 °C
Thermal stability Below 45 °C
Inhibitors Hg2+, Zn2+, Cu2+, Cd2+
Applications For the determination of α-amylase and inorganic phosphate in clinical analysis.
Appearance White lyophilizate
Molecular Weight ca. 34 kDa (Gel filtration)
Notes Expiration date:
Liquid: stable at 37 °C for at least one week
Powder: stable at 30 °C for at least four weeks
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