| Cat# | TRA-013 |
| Specification | 1KU,5KU,20KU |
| Description | Creatine phosphokinase is purified from rabbit skeletal muscle and belongs to the phosphotransferase family, catalyzing the reversible transfer of phosphate between creatine and ATP. |
| Applications | Core component of myocardial infarction diagnostic kits; biomarker detection for muscular diseases; cellular energy metabolism research (CK-creatine phosphate shuttle system); energy monitoring in sports medicine research. |
| CAS | 9001-15-4 |
| Form | White or off-white flakes |
| Activity | 150 U/mg |
| Unit Definition | One unit will transfer 1 μmol of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C. |
| Molecular Weight | 80-86.2 kDa |
| Buffer | 0.25 M glycyl-glycine, pH 7.4: soluble 5.0 mg/mL, clear, colorless to slightly yellow |
| Optimum pH | The forward reaction occurs at a pH of 8.8-9.0, while the reverse reaction occurs at a pH of 6.0-7.0. |
| Endotoxin Level | < 0.1 EU/μg |
| Storage | -20°C |
| Notes | Use 0.1% albumin in reaction buffer to prevent inactivation during dilution. Enzyme activity degrades rapidly in pure water; dissolve using the recommended buffer. |
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