| Cat# | DIA-524 |
| Source | β-Glucosidase: Aspergillus sp. exo-1,3-β-Glucanase: Trichoderma sp. |
| Description | High purity exo-1,3-β-D-glucanase (Trichoderma sp.) + β-glucosidase (Aspergillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| ECNumber | Exo-1,3-β-glucanase: 3.2.1.58 β-glucosidase: 3.2.1.21 |
| CAS No. | exo-1,3-β-glucanase: 9073-49-8 β-glucosidase: 9001-22-3 |
| Optimum pH | 4 |
| Optimum temperature | 40 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | Exo-1,3-β-D-Glucanase: One unit of exo-1,3-β-glucanase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from laminarin (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40 °C. β-Glucosidase: One unit of β-glucosidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from 4-nitrophenyl-β-D-glucpyranoside in sodium acetate buffer (100 mM), pH 4.0 at 40 °C. |
| Storage | 2–8 °C |
| Synonyms | Exo-1,3-β-Glucanase: glucan 1,3-β-glucosidase β-glucosidase: 3-β-D-glucan glucohydrolase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | For use in the determination of (1,3)(1,4) β-glucan. |
| Concentration | Exo-1,3-β-Glucanase: 100 U/mL β-Glucosidase: 20 U/mL |
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