Glucose-1-phosphate thymidyltransferase (EcRmlA) from Escherichia coli mediates the reaction between glucose-1-phosphate and dTTP to produce dTDP-glucose, acts as the first rate-limiting enzyme in the dTDP-L-rhamnose biosynthetic pathway, and supports nucleotide sugar preparation for glycoengineering.
Applications
Glucose-1-phosphate thymidyltransferase (EcRmlA) is used for dTDP-L-rhamnose synthesis detection kits and nucleotide sugar preparation assay reagent development.
One unit is defined as the amount of enzyme that catalyzes the formation of dTDP-Alpha-D-glucose from Alpha-D-glucose 1-phosphate and 1 Mumol dTTP per minute at 37°C.