| Cat# |
TRA-045 |
| Specification |
2KU,10KU,100KU,500KU |
| Description |
Highly active hexokinase suitable for manufacturing high-sensitivity blood glucose detection reagents. |
| Applications |
For R&D and bulk preparation of creatine kinase, creatine kinase isoenzymes, and related reagents. |
| Form |
White to pale yellow amorphous lyophilized powder |
| Source |
Microorganisms |
| Activity |
≥ 45.0 U/mg |
| Unit Definition |
One unit is defined as the amount of enzyme required to generate 1 μmol of NADH per minute under the following reaction conditions. |
| Molecular Weight |
36 kDa (SDS-PAGE) |
| Purity |
≥ 90% |
| Optimum pH |
8 |
| Optimum temperature |
45°C |
| pH Stability |
6.5-9.0 (25°C, 16 h) |
| Thermal Stability |
< 37°C (pH 8.0, 30 min) |
| Isoelectric Point |
5.64 |
| Km Value |
3.2×10^-4 M (D-glucose), 8.3×10^-5 M (ATP) |
| Inhibitors |
SDS, Ag+, Fe3+, Hg2+, Cu2+ |
| Protectant |
BSA |
| Storage |
Store at -25°C to -15°C for 12 months maintains > 90% activity. |
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