| Cat# | ENZD-RT21 |
| Specification | 50 rxns（50 μl/rxn） |
| Aplications | Virus Detection; Gene Expression Analysis; Endpoint PCR Detection |
| Description | Endpoint RT-PCR detection for RNA templates, reverse transcription and PCR completed in one tube, improving throughput and reducing contamination risk. Supports up to 10 kb amplification fragments, in 2× Master Mix form, easy to operate. |
| Features | 1. Reverse transcription and PCR are performed in the same tube. 2. Detection sensitivity can reach 1 pg of total RNA. 3. Can amplify fragments longer than 10 kb. |
| Components | RNase-free ddH2O 2 x One Step Mix One Step Enzyme Mix 10 x DNA loading buffer |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
The ENZD-RT21 one-step RT-PCR system from Creative Enzymes was evaluated for amplification sensitivity and fragment length performance using both cellular and viral RNA templates.
Using HeLa total RNA as template, a 555 bp GAPDH fragment was successfully amplified with a detection sensitivity down to 0.1 pg. In a viral system, PRRSV RNA enabled reliable amplification of a 660 bp target with a detection limit of 0.2 TCID50, demonstrating strong sensitivity across different biological backgrounds. Furthermore, when high-input HeLa RNA (1 μg) was used, the system was able to amplify long fragments up to 12.1 kb (UTRN), indicating robust performance in long-amplicon RT-PCR applications.
These results highlight stable reverse transcription and amplification efficiency across both short and long targets, supported by consistent enzyme performance in complex RNA templates. Data sourced from internal validation studies evaluating RT-PCR sensitivity and long-fragment amplification capability.
