| Cat# | DIA-536 |
| Source | Bacillus licheniformis |
| Description | High purity protease (subtilisin A from Bacillus licheniformis) (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Solution |
| CAS No. | 9014-01-1 |
| Activity | ~ 6 U/mg of protein (40 °C, pH 8.0 on casein) |
| Unit Definition | One unit will hydrolyse casein to produce colour equivalent to one µmole (181 µg) of tyrosine per minute at pH 7.0 at 40 °C. |
| Storage | 2–8 °C |
| Synonyms | Subtilisin; subtilisin A |
| Enzyme Commission Number | EC 3.4.21.62 |
| Stability | > 1 year under recommended storage conditions |
| Optimum temperature | 60 °C |
| Buffer | 50% (v/v) glycerol |
| Molecular Weight | 30250 Da |
| Specificity | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides. |
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