| Cat# | TRA-435 |
| Specification | 5μg(min) |
| Description | Truncated human AMPKα1 was co-expressed as an N-terminal GST fusion protein and PRKAG1 using a baculovirus expression system. The GST-AMPKα1/β1/γ1 complex was purified by glutathione sepharose chromatography and subsequently activated with His-tagged CaMKK1. The activated complex was re-isolated via glutathione sepharose chromatography. |
| Applications | For use as a positive control in AMPK detection assays for metabolic disease research. |
| Synonyms | PRKAA1, AMPK, AMPKa1 |
| Source | Insect (sf21) |
| Class | Serine/Threonine Kinase |
| Group | CAMK |
| Family | CAMKL |
| Gene Name | PRKAA1 | protein kinase AMP-activated catalytic subunit alpha 1 |
| Sequence | 10-559 (end) |
| Accession Number | NP_006242.5 |
| Tag | GST-Tag |
| Tag Location | N-terminal GST |
| Storage Buffer | 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35,1 mM DTT, 10% glycerol, pH 7.5 |
| Storage | Store at -80°C. Avoid repeating freeze-thaws. |
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