| Cat# |
TRA-444 |
| Specification |
10μg(min) |
| Description |
Full-length human AurB with the M298T mutation was co-expressed as an N-terminal DYKDDDDK-tagged, biotinylated protein in a baculovirus expression system. Following purification with DYKDDDDK tag antibody agarose and activation with ATP, the activated complex was isolated by gel filtration. |
| Applications |
For use as a positive control in Aurora B detection assays for chromosomal passenger complex research. |
| Synonyms |
AURKB,AIK2,AIM-1,AIM1,ARK-2,ARK2,AurB,IPL1,PPP1R48,STK-1,STK12,STK5,aurkb-sv1,aurkb-sv2,Aik2 |
| Source |
Insect (sf21) |
| Class |
Serine/Threonine Kinase |
| Group |
Other |
| Family |
AUR |
| Gene Name |
AURKB | aurora kinase B |
| Sequence |
1-344 (end) |
| Accession Number |
NP_004208.2 |
| Tag |
BTN-Tag |
| Tag Location |
N-terminal DYKDDDDK |
| Storage Buffer |
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35,1 mM DTT, 10% glycerol, pH 7.5 |
| Storage |
Store at -80°C. Avoid repeating freeze-thaws. |
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