| Cat# | TRA-541 |
| Specification | 5μg(min) |
| Description | The catalytic domain of human COT was expressed as an N-terminal GST fusion protein using a baculovirus expression system. GST-COT was purified by glutathione sepharose chromatography. The GST tag was removed by PreScission protease cleavage, and the GST-free COT 42 kDa was collected in the flow-through fraction from a subsequent glutathione sepharose chromatography step. |
| Applications | For use as a positive control in COT detection assays for inflammatory signaling research. |
| Synonyms | MAP3K8, AURA2, COT, EST, ESTF, MEKK8, TPL2, Tpl-2, c-COT |
| Source | Insect (sf21) |
| Molecular Weight | 42 kDa |
| Class | Serine/Threonine Kinase |
| Group | STE |
| Family | STE-Unique |
| Gene Name | MAP3K8 | mitogen-activated protein kinase kinase kinase 8 |
| Sequence | 30-397 |
| Accession Number | NP_005195.2 |
| Tag | Tag-free-Tag |
| Tag Location | Tag-free |
| Storage Buffer | 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35,1 mM DTT, 10% glycerol, pH 7.5 |
| Storage | Store at -80°C. Avoid repeating freeze-thaws. |
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