Full-length human PKN3 was co-expressed as an N-terminal GST fusion protein 127 kDa with CDC37 and HSP90α using a baculovirus expression system. GST-PKN3 was purified by sequential glutathione sepharose and gel filtration chromatography.
Applications
For use as a positive control in PKN3 detection assays for cell migration research.
Synonyms
PKN3, UTDP4-1, PKNbeta
Source
Insect (sf21)
Molecular Weight
127 kDa
Class
Serine/Threonine Kinase
Group
AGC
Family
PKN
Gene Name
PKN3 | protein kinase N3
Sequence
1-889 (end)
Accession Number
NP_037487.2
Tag
GST-Tag
Tag Location
N-terminal GST
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35,1 mM DTT, 10% glycerol, pH 7.5