| Cat# | DIA-835 |
| Descriptions | Recombinant Lysyl Edopeptidase is a serine endopeptidase that specifically hydrolyzes peptide bonds at the carboxyl terminus of lysine residues, generates well-defined peptide fragments with high cleavage specificity, and is ideally suited for protein primary structure analysis and proteomics sample preparation. |
| Applications | Recombinant Lysyl Edopeptidase is suitable for protein primary structure detection kits and proteomics sample fragmentation reagent development. |
| Features | 1. No animal origin: no animal diseases, no pathogenic substances, no contamination by exogenous factors, and high safety. 2. Quality stability: This product is a lyophilized powder containing a biologically active protective agent. It has been mass-produced, and the difference between batches is small; the production scale is more than 1000 L. |
| CAS No. | 72561-05-8 |
| Molecular Weight | Approximately 36±3.6 kDa |
| Enzyme Source | Escherichia coli |
| Synonyms | 2-Quinolinepropanoic acid, α-[[[1,2,3,4-tetrahydro-2-[ (phenylmethoxy)carbonyl]-3-isoquinolinyl]carbonyl]amino]-; N-({2-[ (Benzyloxy)carbonyl]-1,2,3,4-tetrahydro-3-isoquinolinyl}carbonyl)-3-(2-quinolinyl)alanine |
| Activity | ≥ 3.0 U/mg |
| Activity Definition | At 30°C | pH 9.5 | the amount of enzyme that produces 1 μmol of p-nitroaniline per minute for the catalytic substrate is 1 AU. |
| Storage | It is transported in an ice pack to keep it active. It is transported in an ice pack to keep it active. Store at -15~-20°C, stable within 24 months. |
| Usage & Dosage | 1. Protein preparation. The protein was dissolved in a solution of 25 mM Tris-HCl, pH 9.2. Proteins with low solubility or denaturation treatment can be solubilized by incubation with a minimum volume of denaturing agent such as 8 M urea or 6 M guanidine hydrochloride at 25- 37°C for 1 h. Proteins that require disulfide reduction treatment can be added to DTT or β-mercaptoethanol at a final concentration of 5 mM and incubated at 50-60°C for 20 minutes to dissolve. The volume of the reaction solution was adjusted so that Tris-HCl was 25 mM, urea was 1 M or less, and guanidine hydrochloride was 0.1 M or less. 2. Enzyme preparation. The desired enzyme powder was weighed and dissolved in 25 mM Tris-HCl (pH 9.2) to give an enzyme solution concentration of 1-10 mg/ml. 3. Digestive response. The amount of 1 g of polypeptide or protein recommended enzyme is between 1 and 100 AU. It is recommended to react at 25-37°C for 2-18 h. If it is necessary to terminate the reaction, acetic acid or hydrochloric acid may be added to adjust the pH to acidity. |
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