| Cat# | DIA-585 |
| Source | Escherichia coli |
| Description | T4 DNA polymerase catalyzes DNA synthesis in the 5′→3′ direction in the presence of template and primers. It possesses strong 3′→5′ exonuclease activity, which is greater than that of DNA polymerase I, but lacks 5′→3′ exonuclease activity. These properties make it suitable for applications such as end polishing and blunt-end formation in molecular biology workflows. |
| Unit Definition | One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material within 30 minutes at 37 °C. |
| Storage | -20 °C |
| Buffer | 10 mM Tris-HCl pH 7.4, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol |
| Applications | Gap filling; removal of 3′ overhangs or filling-in of 5′ overhangs to generate blunt ends. |
| Product Overview | Gap filling (without strand displacement activity). Removal of 3′ overhangs or filling in of 5′ overhangs to generate blunt ends. |
| Package | 2000 U T4 DNA polymerase (3 U/μL): 667 μL 10× Blue Buffer (10× Blue Buffer; 100 mM Tris-HCl pH 7.9 25 °C; 500 mM NaCl; 100 mM MgCl2; 10 mM DTT): 2 mL |
| Download Datasheet: |  |
