| Cat# | DIA-577 |
| Unit Definition | One unit of enzyme activity is defined as the amount of enzyme required to incorporate 1 nmol of AMP into polynucleotides in 1 hour at 37 °C and pH 8.0. |
| Storage | -20 °C |
| Buffer | 50 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% (v/v) Triton X-100, 50% (v/v) glycerol |
| Product Overview | 5′→3′ RNA polymerase activity. Supports synthesis of labeled or unlabeled RNA. Compatible with nucleotide substrates modified with biotin, digoxigenin, or fluorescent tags for RNA labeling. |
| Notes | Perform all handling and transcription reactions in an RNase-free environment. Wearing gloves and adding RNase inhibitors to the reaction are recommended to prevent RNA degradation. For transcripts shorter than 100 nt, the template amount can be increased up to 2 µg. To avoid precipitation caused by DNA and spermidine interaction at low temperatures, prepare the reaction mix at room temperature. |
| Package | 2/10/100 KU |
| Download Datasheet: |  |
