Alkaline phosphatase (Apase) is a widely applied hydrolase active under alkaline conditions, capable of catalyzing the hydrolysis of various phosphate esters to release inorganic phosphate, with stable activity for molecular labeling and biochemical detection.
Applications
Alkaline phosphatase (Apase) is widely used as labeling enzyme raw material for immunoassay kits and nucleic acid detection related reagent development.
CAS No.
131-3-11
Enzyme Commission Number
EC 3.1.3.1
Enzyme Source
E.coli recombinant alkaline phosphatase from Shewanella.sp.
Reaction
AMP = Adenosine + Pi
Activity Definition
One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol adenine from AMP per minute at 37°C.