Beta-Galactosidase (EcLacZ) encoded by the E. coli lacZ gene catalyzes the hydrolysis of lactose into glucose and galactose, features well-characterized enzymatic properties, and is the most widely used reporter enzyme in molecular biology and gene expression research.
Applications
Beta-Galactosidase (EcLacZ) serves as a classic reporter enzyme for gene expression detection kits and molecular biology assay reagent development.
CAS No.
32-1-232
Enzyme Commission Number
EC 3.2.1.23
Enzyme Source
E.coli recombinant Beta-galactosidase from E.coli.
Reaction
GalBeta1, 4Glc = Gal+Glc
Activity Definition
One unit is defined as the amount of enzyme that catalyzes the hydrolysis of 1 μmol GalBeta1,4Glc per minute at 37°C.