| Cat# | DIA-690 |
| Descriptions | Diaphorase is a flavin-dependent oxidoreductase that catalyzes the oxidation of NADH or NADPH to reduce various electron acceptors, is widely utilized in coenzyme regeneration systems and biochemical detection kits, and supports various oxidoreductase-coupled reaction systems. |
| Applications | Diaphorase is widely applied in coenzyme regeneration detection kits and biochemical detection kit auxiliary reagent development. |
| CAS No. | 9001-18-7 |
| Enzyme Commission Number | EC 1.6.5.2 |
| Enzyme Source | Recombinant expressed by E.coli. |
| Activity Definition | One unit is defined as the amount of enzyme which oxidizes one micromole NADH per minute under the conditions of pH7.0 at 37°C as described.The activity concentration (U/L) is calculated by detecting ∆A/min of formazane dye production rate at 550nm optical wavelength. Millimolar extinction coefficient of formazane dye under the assay conditions is 12.4 cm2/micromole. |
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