| Cat# | DIA-469 |
| Description | A deoxyribonuclease that randomly cleaves ssDNA and dsDNA, producing 5′-phosphate oligonucleotides. Activity depends on Ca2+ and is activated by Mg2+ or Mn2+. Glycerol-free, suitable for lyophilization. |
| Unit Definition | One unit (U) is defined as the amount of enzyme required to completely degrade 1 μg of pBR322 plasmid DNA in 10 minutes at 37 °C. |
| Storage | –20 °C |
| Synonyms | Deoxyribonuclease I |
| Contaminants | Free of RNase contamination |
| Applications | Preparation of DNA-free RNA samples; removal of genomic DNA contamination prior to RT-PCR; elimination of DNA templates after in vitro transcription with T7, T3, or SP6 RNA polymerases; footprinting assays for DNA–protein interaction analysis; nick translation when combined with DNA polymerase I; DNA fragmentation to generate random libraries in the presence of Mn2+; positive control for DNA fragmentation in apoptosis TUNEL assays. |
| Product Overview | Product Components: 200 U: DNase I (Glycerol-free, 1 U/μL): 200 μL 10× Reaction Buffer with MgSO4: 1 mL 25 mM EDTA: 1 mL 2000 U: DNase I (Glycerol-free, 1 U/μL): 2 mL 10× Reaction Buffer with MgSO4: 10 mL 25 mM EDTA: 10 mL 20 KU: DNase I (Glycerol-free, 1U/μl): 20 mL 10× Reaction Buffer with MgSO4: 100 ml 25mM EDTA: 100 mL 50 KU: DNase I (Glycerol-free, 1 U/μL): 50 mL |
| Package | 200 U/2000 U/20 KU/50 KU |
| Download Datasheet: |  |
