| Cat# | DIA-421 |
| Source | Recombinant Tritirachium album |
| Description | Proteinase K is a highly stable serine protease with broad substrate specificity. It efficiently degrades native proteins even in the presence of detergents, making it ideal for nucleic acid purification. Belonging to the subtilisin family, it cleaves peptide bonds next to aliphatic or aromatic amino acids. Its robustness and wide activity range make it a standard tool in molecular biology. |
| Form | Freeze dried powder |
| Activity | ≥ 30 U/mg |
| Isoelectric point | 7.81 |
| Storage | Below 8 °C |
| Enzyme Commission Number | EC 3.4.21.64 |
| pH Stability | pH 4.5–12.5 (25 °C, 16 h) |
| Optimum pH | 7.0–12.0 |
| Optimum temperature | 65 °C |
| Thermal stability | Below 50 °C (pH 8.0, 30 min) |
| Inhibitors | Diisopropylfluorophosphate, phenylmethylsulfonylfluoride |
| Applications | Genetic diagnostic kit; RNA and DNA extraction kits; Extraction of non-protein components from tissues, degradation of protein impurities, such as DNA vaccines and preparation of heparin; Preparation of chromosome DNA by pulsed electrophoresis; Western blot; Enzymatic glycosylated albumin reagents in vitro diagnosis. |
| Appearance | White to off-white amorphous lyophilizate |
| Molecular Weight | 29 kDa (SDS-PAGE) |
| Warnings | Wear protective gloves and goggles when using or weighing, and keep well ventilated after use. This product may cause skin allergic reaction and serious eye irritation. If inhaled, it may cause allergy or asthma symptoms or dyspnea. |
| Package | 100 mg/1 g/10 g/100 g |
| Download Datasheet: |  |
