Endo-β-N-acetylglucosaminidase S2 (Endo S2) from Streptococcus pyogenes exhibits broad substrate specificity for all major types of N-linked glycans, cleaves the chitobiose core of N-glycans while leaving a single GlcNAc on the protein, and is a premium tool for antibody glycosylation analysis and remodeling.
Applications
Endo-β-N-acetylglucosaminidase S2 (Endo S2) is widely used in antibody glycosylation detection kits and N-glycan profiling assay reagent development.
Enzyme Commission Number
EC 3.2.1.96
Activity Definition
The unit enzyme activity is defined as the amount of enzyme required to remove more than 95% of carbohydrates from 5 μg of human IgG in a 10 μl reaction system for 1 hour at 37°C under the following conditions.