| Cat# | ENZD-RT09 |
| Specification | 100 rxns 500 rxns |
| Aplications | LAMP/RT-LAMP Isothermal Amplification |
| Description | Provides 2× fluorescent LAMP/RT-LAMP Master Mix, supports real-time fluorescent detection of DNA or RNA templates. Integrates Bst II Pro enzyme and RTv reverse transcriptase, possessing both high specificity and stability for room temperature preparation. |
| Quality Control | Excellent LAMP & RT-LAMP amplification performance. High template compatibility. Supports system establishment at room temperature. |
| Components | 2 × Fluorescent LAMP/RT-LAMP Master Mix 50 × LAMP Fluorescent Dye |
| Shipping and Storage Conditions | Store at -30 ~ -15°C, transport at ≤0°C |
| Download Datasheet: |  |
The ENZD-RT09 isothermal amplification premix from Creative Enzymes was evaluated across multiple LAMP and RT-LAMP systems under varying template concentrations and compared with a commercial reagent.
ENZD-RT09 demonstrated superior LAMP & RT-LAMP amplification performance, with consistently faster amplification kinetics and reliable detection across different systems, indicating strong efficiency and robustness. Data sourced from internal validation studies evaluating amplification performance across multiple LAMP and RT-LAMP systems.
ENZD-RT09 was tested using crude lysates derived from human whole blood, saliva, and buccal swab samples to assess template compatibility.
The premix maintained stable amplification across all sample types and input levels, demonstrating high tolerance to complex biological matrices and consistent performance under varying conditions. Data sourced from internal validation studies assessing compatibility with crude sample inputs across multiple biological sources.
Reaction systems containing templates were analyzed immediately after preparation or after incubation at room temperature for 3 hours.
No significant differences in positive amplification were observed after room-temperature incubation, and NTC controls showed lower background compared to the commercial reagent, confirming stable performance and strong specificity. Data sourced from internal validation studies evaluating amplification stability and background control after room-temperature incubation.
