| Cat# | DIA-435 |
| Description | A FAD-dependent glutamate oxidase with high specificity for glutamate. It is produced using genetic recombination technology to ensure consistent quality. |
| Form | Freeze dried powder |
| Activity | ≥ 15 U/mg lyophilizate |
| Unit Definition | One unit (U) is defined as the amount of enzyme which produces 1 μmol of hydrogen peroxide per min at 30 °C and pH 7.4 under the conditions described below. |
| Enzyme Commission Number | EC 1.4.3.11 |
| pH Stability | 6.0–8.5 |
| Michaelis Constant | 3.2 × 10-4 M (L-glutamate) |
| Optimum pH | 6.0–8.0 |
| Optimum temperature | 40–50 °C |
| Thermal stability | Below 55 °C |
| Applications | Glutamate can be quantified by colorimetry using glutamate oxidase and Trinder's reagent. This product can also be immobilized on an electrode to continuously measure glutamate using the electrode method. |
| Appearance | Light yellow to yellow lyophilizate |
| Molecular Weight | ca. 115 kDa (Gel filtration) |
| Notes | Expiration date: Liquid: stable at 37 °C for at least two weeks Powder: stable at 30 °C for at least one month |
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