| Cat# | BDE-014 |
| Description | Creatinine Amidohydrolase specifically hydrolyzes creatinine and serves as the key initiating enzyme in the enzymatic assay for creatinine. |
| Applications | Used in creatinine enzymatic assay kits to specifically hydrolyze creatinine into creatine. |
| CAS No. | 9025-13-2 |
| Enzyme Commission Number | EC 3.5.2.10 |
| Form | White to pale yellow amorphous lyophilized powder |
| Source | Microorganism |
| Activity | ≥ 400.0 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the production of 1 μmol of creatine per minute under specified assay conditions. |
| Molecular Weight | 29 kDa (SDS-PAGE) |
| Purity | ≥ 90% by SDS-PAGE |
| Optimum pH | 7.0-8.0 |
| Optimum temperature | 65°C |
| pH Stability | 5.5-10.0 (25°C, 16 h) |
| Thermal Stability | < 65°C (pH 8.0, 30 min) |
| Isoelectric Point | 5.3 |
| Km Value | 5.0×10^2 M (Creatinine), 8.0×10^2 M (Creatine) |
| Inhibitors | Hg2+, Cu2+, Fe3+ |
| Protectant | Carbohydrates and BSA |
| Storage | Store at -25°C to -15°C for 12 months to maintain at least 90% activity. |
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