Glycerokinase from Cellulomonas sp.
| Cat# | NATE-0287 |
| Abbr | GK, Native (Cellulomonas sp.) |
| Alias | GK |
| Source | Cellulomonas sp. |
| Description | Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate. |
| Applications | This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate oxidase or pyruvate kinase and lactate dehydrogenase, lipoprotein lipase in clinical analysis. |
| Product Overview | The enzyme has the highest specificity for glycerol, and also phosphorylates dihydroxyacetone and glyceraldehyde. Mg++ is essentially required for the reaction. |
| Form | Lyophilized powder containing phosphate buffer salts and sodium gluconate |
| Enzyme Commission Number | EC 2.7.1.30 |
| Activity | 20 U/mg-solid or more |
| CAS No. | 9030-66-4 |
| Isoelectric point | 4.2 |
| pH Stability | pH 5.5 x 10.0 (25°C, 20hr) |
| Michaelis Constant | 4.4 x 10-5M (Glycerol), 4.3 x 10-4M (ATP) |
| Unit Definition | One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25°C in a coupled system with PK/LDH. |
| Optimum pH | 9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system) |
| Optimum temperature | 50°C |
| Thermal stability | below 40°C (pH 7.5, 15min) |
| Storage | −20°C |
| Inhibitors | p-Chloromercuribenzoate, heavy metal ions (Pb++, Fe++, Hg++, Ag+) |
| Synonyms | EC 2.7.1.30; glycerokinase; GK; ATP:glycerol-3-phosphotransferase; glycerol kinase (phosphorylating); glyceric kinase; 9030-66-4 |
Download Datasheet:
|
