diagnostic-enzymes

Glycerokinase from Cellulomonas sp.

Glycerokinase from Cellulomonas sp.

Cat# AbbrAliasSimilarSourceDescriptionApplicationsProduct OverviewFormEnzyme Commission NumberActivityCAS No. Isoelectric pointpH StabilityMichaelis ConstantUnit DefinitionOptimum pHOptimum temperatureThermal stabilityStorageInhibitorsSynonyms
NATE-0287 GK, Native (Cellulomonas sp.)GKGlycerokinaseCellulomonas sp.Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate oxidase or pyruvate kinase and lactate dehydrogenase, lipoprotein lipase in clinical analysis.The enzyme has the highest specificity for glycerol, and also phosphorylates dihydroxyacetone and glyceraldehyde. Mg++ is essentially required for the reaction.Lyophilized powder containing phosphate buffer salts and sodium gluconateEC 2.7.1.3020 U/mg-solid or more9030-66-44.2pH 5.5 x 10.0 (25°C, 20hr)4.4 x 10-5M (Glycerol), 4.3 x 10-4M (ATP)One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25°C in a coupled system with PK/LDH.9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system)50°Cbelow 40°C (pH 7.5, 15min)−20°Cp-Chloromercuribenzoate, heavy metal ions (Pb++, Fe++, Hg++, Ag+)EC 2.7.1.30; glycerokinase; GK; ATP:glycerol-3-phosphotransferase; glycerol kinase (phosphorylating); glyceric kinase; 9030-66-4 Download Datasheet:
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