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HotStart Taq DNA Polymerase (Glycerol-free)

Cat# DIA-442
Description Taq polymerase chemically treated to block activity before heating, preventing low-temperature non-specific amplification. Suitable for high-specificity PCR, multiplex PCR, high-GC content (>60%), and complex genomic amplification. Glycerol-free, lyophilization compatible.
Unit Definition One unit (U) is defined as the amount of enzyme required, after heat activation, to incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 74 °C.
Storage –20 °C
Contaminants Free of E. coli DNA residues; free of endo-/exonuclease contamination
Applications High-specificity PCR; amplification of complex templates; multiplex PCR; genome amplification assays; qPCR.
Product Overview Product Components:
250 U:
HotStart Taq DNA Polymerase (Glycerol-free, 5 U/μL): 50 μL
5× HS Taq Buffer with Mg2+: 1 mL × 2
2500 U:
HotStart Taq DNA Polymerase (Glycerol-free, 5 U/μL): 500 μL
5× HS Taq Buffer with Mg2+: 10 mL × 2
500 U:
HotStart Taq DNA Polymerase (Glycerol-free, 50U/μl): 10 μL
5× HS Taq Buffer with Mg2+: 2 mL × 2
Package 250/2500/500 U
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