| Cat# |
DIA-442 |
| Description |
Taq polymerase chemically treated to block activity before heating, preventing low-temperature non-specific amplification. Suitable for high-specificity PCR, multiplex PCR, high-GC content (>60%), and complex genomic amplification. Glycerol-free, lyophilization compatible. |
| Unit Definition |
One unit (U) is defined as the amount of enzyme required, after heat activation, to incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 74 °C. |
| Storage |
–20 °C |
| Contaminants |
Free of E. coli DNA residues; free of endo-/exonuclease contamination |
| Applications |
High-specificity PCR; amplification of complex templates; multiplex PCR; genome amplification assays; qPCR. |
| Product Overview |
Product Components: 250 U: HotStart Taq DNA Polymerase (Glycerol-free, 5 U/μL): 50 μL 5× HS Taq Buffer with Mg2+: 1 mL × 2 2500 U: HotStart Taq DNA Polymerase (Glycerol-free, 5 U/μL): 500 μL 5× HS Taq Buffer with Mg2+: 10 mL × 2 500 U: HotStart Taq DNA Polymerase (Glycerol-free, 50U/μl): 10 μL 5× HS Taq Buffer with Mg2+: 2 mL × 2 |
| Package |
250/2500/500 U |
| Download Datasheet: |
 |