| Cat# | DIA-471 |
| Description | A nuclease that cleaves DNA phosphodiester bonds, producing 5′-phosphate and 3′-hydroxyl oligonucleotides. Highly active and heat-labile; preferentially degrades dsDNA (≥ 5000× more active on dsDNA than ssDNA) while preserving ssDNA and RNA. Inactivated irreversibly after 5 min at 55 °C. Compatible with M-MLV and AMV reaction buffers, ideal for removing genomic DNA contamination in reverse transcription reactions. |
| Unit Definition | One unit (U) is defined as the amount of enzyme that increases the absorbance at 260 nm by 0.001 OD per minute under the assay conditions of high-molecular-weight dsDNA substrate at 25 °C and pH 5.0. |
| Storage | –20 °C |
| Inhibitors | Metal chelators, transition metals, SDS, and reducing agents |
| Applications | Preparation of RNA or ssDNA samples free of dsDNA; removal of genomic DNA contamination from RNA samples; removal of DNA templates after in vitro RNA transcription. |
| Product Overview | Product Components: 100 U: HL-dsDNase (2 U/μL): 50 μL 10× HL-dsDNase Buffer: 1 mL 20× Stop Solution: 100 μL 1000 U: HL-dsDNase (2 U/μL): 500 μL 10× HL-dsDNase Buffer: 1 mL x 2 20× Stop Solution: 1 mL Product Features: Double-stranded DNA-specific endonuclease Strong activity within 20–47 °C range Heat inactivatable Preserves RNA/protein activity at low temperatures without affecting RNA or ssDNA quality |
| Package | 100/1000 U |
| Download Datasheet: |  |
