| Cat# | DIA-532 |
| Source | Flavobacterium odoratum |
| Description | Pure isoamylase (glycogen 6-glucanohydrolase) from Flavobacterium odoratum for use in research, biochemical enzyme assays and analytical testing applications. |
| Form | Suspension |
| CAS No. | 9067-73-6 |
| Activity | ~ 140 U/mg (40 °C, pH 6.0 with 1 mM CaCl2 on oyster glycogen) |
| Unit Definition | One unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10 mg/mL) in MES buffer (100 mM, pH 6.0) plus 1 mM CaCl2 at 40 °C. |
| Storage | 4 °C |
| Synonyms | Isoamylase; glycogen 6-α-D-glucanohydrolase |
| Enzyme Commission Number | EC 3.2.1.68 |
| Stability | > 1 year under recommended storage conditions |
| Optimum temperature | 50 °C |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications in carbohydrate research and in the food and feeds, and cereals industry. |
| Molecular Weight | 83000 Da |
| Concentration | ~ 200 U/mL |
| Specificity | Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins. |
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