| Cat# | DIA-544 |
| Source | Prokaryote |
| Description | High purity recombinant succinyl-CoA synthetase from prokaryote for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| CAS No. | 9080-33-5 |
| Activity | ~ 9 U/mg (25 °C, pH 8.4 on succinic acid) |
| Unit Definition | One unit of succinyl-CoA synthetase activity is defined as the amount of enzyme required to release one μmole of succinyl CoA from succinic acid (5.8 mM) per minute in the presence of NADH and Coenzyme A in Glycylglycine buffer (34 mM), pH 8.4 at 25 °C. |
| Storage | 2–8 °C |
| Synonyms | Succinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming) |
| Enzyme Commission Number | EC 6.2.1.5 |
| Stability | > 1 year under recommended storage conditions |
| Optimum pH | 8.4 |
| Optimum temperature | 25 °C |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications for the measurement of succinic acid in the clinical chemistry, food, wine and dairy industries. |
| Concentration | ~ 220 U/mL |
| Specificity | Catalyses the reaction: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA. |
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