| Cat# | ENZD-RT04 |
| Specification | 10000 U |
| Unit Concentration | 200 U/μl |
| Description | Third-generation reverse transcriptase with high impurity tolerance and higher efficiency. This product is a next-generation reverse transcriptase that can perform highly efficient reverse transcription reactions at 37°C. It retains the thermostability of the second-generation product, and for RNA with complex secondary structures, the reverse transcription temperature can be increased to 50°C-55°C, avoiding the inhibition of cDNA synthesis by the complex secondary structure of RNA, thus effectively synthesizing high-quality cDNA. Furthermore, this product still exhibits excellent sustained synthesis capability and strong impurity tolerance. |
| Features | • Broad template compatibility: Compatible with various templates including animal, plant, and viral samples. • Excellent impurity tolerance: Exhibits superior tolerance to common impurities (ethanol, isopropanol, water-balanced phenols, humic acid). • Superior reverse transcription efficiency: Demonstrates higher reverse transcription efficiency compared to commercially available reverse transcriptases. |
| Applications | Reverse transcription; first-strand cDNA synthesis; two-step qRT-PCR detection; cDNA library construction |
| Components | 5 x High Buffer III High Reverse Transcriptase III (200 U/µl) |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
Using 1 μg of HeLa total RNA as input, reverse transcription was performed following the recommended protocol for ENZD-RT04. Subsequently, 1 μL of the synthesized cDNA was used as a template for long-fragment amplification.
Agarose gel electrophoresis analysis showed a single, well-defined amplification band with high yield, indicating efficient cDNA synthesis and excellent template integrity. As a high-efficiency reverse transcriptase, ENZD-RT04 demonstrates strong impurity tolerance and broad template compatibility, ensuring consistent performance across complex RNA samples and supporting reliable amplification of long nucleic acid fragments with high specificity and purity.
Data generated from internal validation studies using HeLa RNA templates, demonstrating consistent amplification performance.
