| Cat# |
TRA-438 |
| Specification |
10μg(min) |
| Description |
Truncated human AMPKα1 was co-expressed as an N-terminal DYKDDDDK-tagged, biotinylated protein and PRKAG1 using a baculovirus expression system. The complex was isolated via DYKDDDDK tag antibody agarose, activated with His-tagged CaMKK1, and the activated complex was re-purified using DYKDDDDK tag antibody agarose. |
| Applications |
For use in comprehensive AMPK isoform-specific detection panels. |
| Synonyms |
PRKAA1, AMPK, AMPKa1 |
| Source |
Insect (sf21) |
| Class |
Serine/Threonine Kinase |
| Group |
CAMK |
| Family |
CAMKL |
| Gene Name |
PRKAA1 | protein kinase AMP-activated catalytic subunit alpha 1 |
| Sequence |
10-559 (end) |
| Accession Number |
NP_006242.5 |
| Tag |
BTN-Tag |
| Tag Location |
N-terminal DYKDDDDK |
| Storage Buffer |
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35,1 mM DTT, 10% glycerol, pH 7.5 |
| Storage |
Store at -80°C. Avoid repeating freeze-thaws. |
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