Nicotinamide adenine dinucleotide (NADH) oxidases (SmNox) derived from Streptococcus mutans catalyze the oxidation of NADH to NAD+ with concomitant reduction of molecular oxygen to water, serve as an efficient tool for NAD+ regeneration in coupled enzymatic reactions, and effectively improve the economy of industrial biocatalytic processes.
Applications
Nicotinamide adenine dinucleotide (NADH) oxidases (SmNox) serve as core components for NAD+ regeneration systems in biochemical detection kits and coupled enzymatic assay reagent development.
CAS No.
16-99-3
Enzyme Commission Number
EC 1.6.99.3
Enzyme Source
E.coli recombinant Sm NOX from Streptococcus mutans.
Reaction
NAD++O2=NADH+H2O2
Activity Definition
One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of NADH from NAD+ at 37°C.