| Cat# | TRA-452 |
| Specification | 5μg(min) |
| Description | The catalytic domain of human BRAF was expressed as an N-terminal GST fusion protein 60 kDa using a baculovirus expression system. GST-BRAF was purified by glutathione sepharose chromatography. |
| Applications | For use as a positive control in BRAF mutation detection assays for melanoma and colorectal cancer companion diagnostics. |
| Synonyms | BRAF, B-RAF1, B-raf, BRAF1, NS7, RAFB1 |
| Source | Insect (sf21) |
| Molecular Weight | 60 kDa |
| Class | Serine/Threonine Kinase |
| Group | TKL |
| Family | RAF |
| Gene Name | BRAF | B-Raf proto-oncogene, serine/threonine kinase |
| Sequence | 433-726 |
| Accession Number | NP_004324.2 |
| Tag | GST-Tag |
| Tag Location | N-terminal GST |
| Storage Buffer | 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35,1 mM DTT, 10% glycerol, pH 7.5 |
| Storage | Store at -80°C. Avoid repeating freeze-thaws. |
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